Flottilin antibody

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August undefined, 2024

WebJul 3, 2024 · Blots were probed with primary antibodies against positive (caveolin and flotillin) and negative (clathrin and Na + /K + ATPase) lipid raft markers in addition to NTCP and OCT1 overnight at 4 °C in the respective blocking solution. Blots were washed three times with TBS-T before incubation with HRP-conjugated secondary antibodies in 2.5% … WebFlotillin-1 Antibody Summary. The immunogen recognized by this antibody maps to a region between residue 377 and 427 of human …

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WebFeb 24, 2024 · Datasheets. Flotillin-1 Antibody (C-7) is a mouse monoclonal IgG 1 κ, cited in 17 publications, provided at 200 µg/ml. raised against amino acids 324-427 of Flotillin-1 of human origin. Anti-Flotillin-1 Antibody (C-7) is recommended for detection of Flotillin-1 of mouse, rat and human origin by WB, IP, IF and ELISA. WebAll lanes : Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/1000 dilution Lane 1 : Wild-type HEK293T cell lysate Lane 2 : FLOT1 knockout HEK293T cell lysate Lane 3 : A431 cell lysate Lane 4 : HAP1 cell lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed at 1/10000 dilution Predicted … real black oled

Anti-Flotillin 1 antibody KO Tested (ab41927) Abcam

WebAnti-Flotillin 1 antibodies are available from several suppliers. In humans, this protein is encoded by the gene FLOT1. The protein may also be known as integral membrane component of caveolae. The expected protein mass is 47.4 kDa, but there are 2 reported isoforms. Variants of this antigen may also be found in other species, including plant ... WebThe blot was probed with Flotillin 2 Polyclonal Antibody (Product # PA5-30033, 1:500 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 … how to tap steel

Flotillin 2 antibody Western, Immunofluorescence F1680 Anti-ESA

Anti Flotillin-1 Antibody (PrecisionAb Polyclonal Antibody) - Bio …

WebMar 8, 2024 · ab247493 is the carrier-free version of ab78178. Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency. This conjugation-ready format is designed for use with fluorochromes, metal ... WebHuman Flotillin-1 recombinant protein corresponding to amino acids 1-270 expressed in E. coli. Formulation. This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives. Preparation. The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions. how to tap real aleWebImmunofluorescence staining of Jurkat cells with purified ab133497 at a working dilution of 1/500, counter-stained with DAPI. The secondary … how to tap on bass guitar

"WebDescription. Flotillin 1 antibody LS-B9180 is an unconjugated goat polyclonal antibody to Flotillin 1 (FLOT1) (aa415-427) from human. It is reactive with human, bovine, dog and other species. Validated for IHC, Peptide-ELISA … " - Flottilin antibody

Flottilin antibody

IHC-plus™ FLOT1 / Flotillin 1 Polyclonal Antibody Goat LSBio

WebImmunofluorescence staining of Jurkat cells with purified ab133497 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was Alexa Fluor ® 488 goat anti-rabbit (), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor ® 594 goat anti-mouse, … WebFlotillin-1, encloded by the FLOT1 gene, is a caveolae-associated, integral membrane protein. The function of flotillin-1 has not been determined (provided by RefSeq, Jul 2008). Goat anti Human flotillin-1 antibody detects a band of 47 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

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WebIn addition to its colocalization with lipid rafts on the plasma membrane, flotillin-1 also has been found in compartments of the endocytic and … WebDec 11, 2005 · Antibodies against flotillin-1, Shp2 (a cytosolic protein) and ERp72 (an endoplasmic reticulum membrane protein) were used separately; specificity of the flotillin-1 antibody was confirmed in ...

WebWestern blot of anti-Flotillin-1 antibody (clone W16108A) and isotype-matched IgG2a control. Blots were incubated with 5µg/ml (left) or 0.5µg/ml (middle) anti-Flotillin-1 antibody, or rat IgG2a, κ isotype control antibody (right) overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-rat secondary antibody. WebMonoclonal Antibody for studying flotillin-1. Cited in 71 publications. Validated for WB, IP, IHC, IF. Available in 2 sizes. Highly specific and …

WebOct 4, 2024 · Monoclonal antibodies are designed in a lab to attack a specific part of a virus. Dr. M.G. Finn, of Georgia Tech , points out that laboratory created antibodies … WebRecombinant Anti-Flotillin 1 antibody [EPR6041] ab133497 is a primary antibody from Abcam. 57 Citations Host: Rabbit Clonality: Recombinant (EPR6041) Validations: Knockout. Applications: WB, FC/FACS and 5 more Reactivity: Homo sapiens (Human), Mus musculus (House mouse), and Rattus norvegicus (Rat)

WebJan 16, 2024 · Flotillin-2 Antibody (B-6) is a mouse monoclonal IgG 1 κ Flotillin-2 antibody, cited in 57 publications, provided at 200 µg/ml. raised against amino acids 151 …

WebHuman Flotillin-1 recombinant protein corresponding to amino acids 1-270 expressed in E. coli. Formulation. This antibody is provided in 50% glycerol in aqueous buffered … how to tap strafe off a jump padWebRed blood cell antibody identification: Single antibody specificity; Multiple antibody specificities; Antibody to high prevalence antigen; Antibody to low prevalence antigen; … how to tap strafe more consistentlyWebFlotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites. However, overexpressed flotillin-2 has also been found in the late endosome (4,8,9). Both flotillin-1 and flotillin-2 are … real black pearls costWebAnti-Flotillin 1-CY3 ™ antibody is suitable for direct immunofluorescence at a concentration of 2-5μg/mL using human HEK-293T or HeLa cells. Biochem/physiol Actions. Flotillin 1 is directly connected to the plasma membrane. The association of flotillin 1 depends on the palmitoylation in a conserved Cys34 residue, whereas the association of ... real black princessesWebFlotillin 1 Antibody (MA5-35364) in WB. Western Blot analysis of Flotillin 1 performed on various cell lines using a Flotillin 1 monoclonal antibody (Product # MA5-35364) at a dilution of 1:1,000. An HRP Goat Anti … how to tap stainless steelWeb"Flotillin 1 antibodies" comparison. At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs. real black pumpkins for saleWebGeneral notes. This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility. - Improved sensitivity and specificity. - Long-term security of supply. - Animal-free production. For more information see here. Our RabMAb ® technology is a patented hybridoma ... how to tap strafe on controller steam